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. Molecular diagnostic assays for detection of SVDV infection. The Vesicular exanthema of swine virus (VESV, Caliciviridae, Vesivirus) can also cause vesicular disease in pigs. The origin of VES was traced to feeding meat from sea mammals to pigs and was eradicated from California populations i...

mPCR is based on traditional PCR, whereby multiple pairs of specific primers and templates are added into the same PCR reaction system (primers are specifically bound to corresponding templates) to amplify multiple DNA fragments with different sequences (Zhang et al., 2020c;Hossain et al., 2021...

4Osaka University Hospital, Osaka University, Osaka, Japan. 5Faculty of Veterinary Medicine, University of Montreal, Québec, Canada. PMID:28666619 DOI:10.1016/j.mcp.2017.06.007 Abstract A multiplex polymerase chain reaction (mPCR) was developed for simultaneous detection (single reaction) of genes ...

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After standardization, its sensitivity and specificity were evaluated on samples from 118 TB suspected and 31 non-TB patients and compared it with smear, culture and mPCR methods. RESULTS: LAMP was able to detect 5 fg DNA (one MTB) within 21 min and found to be 10 times more sensitive ...