[YPD-008]发行于2010-08-21时长41分钟出品商是C-Format,YPD-008作品种子搜索下载,[NGKS-028]发行于2010-07-22时长161分钟出品商是人間考察,NGKS-028作品种子搜索下载
[YPD-008]发行于2010-08-21时长41分钟出品商是C-Format,YPD-008作品种子搜索下载,[NGKS-028]发行于2010-07-22时长161分钟出品商是人間考察,NGKS-028作品种子搜索下载
cellular morphologies of the indicated strains after being grown on ypd plates for 3 days at 30°c. scale bar: 10µm. https://doi.org/10.1371/journal.pone.0059887.g008 discussion we report the ura4 deletion strains of s. pombe and s. japonicus undergo dramatic cell lysis when grown on...
liangli_008 可以用，我做了很久。2010-06-01IP重庆重庆 收藏回复点赞
Fungal strains were grown on different culture media: 2% malt-agar, Yeast Potato Dextrose (YPD) (1% yeast extract, 2% peptone, 2% dextrose), Glc-MM, GlcNAc-MM (MM in which Glc was replaced by GlcNAc), Brian (Brian et al., 1961), RPMI (Invitrogen) buffered with 34.53 g/L of MOPS ...
Transformed cells were incubated in 0.5 mL YPD during 1 h, after which they were re-suspended in 100 µL of sterile demi-water and plated on selective medium. Strains were re-streaked from the transformation plates twice prior to storage of single colony isolates. Strain IMI350 (SeCEN6:...
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ura3Δ0 YOR245C∷kanMX4), the dga1Δ lro1Δ double-mutant strain (MATa his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0 YOR245C∷kanMX4 YNR008W∷kanMX4), and the dga1Δ lro1Δ yju3Δ triple-mutant strain (MATa his3Δ1 leu2Δ0 ura3Δ0 YOR245C∷kanMX4 YNR008W∷kanMX4 YKL094W∷kan...